Calcium ions inhibited streaming in detached leaves, whereas magnesium ions stimulated the streaming rate. We plugged the cylinders with stoppers and waited 48 hours to see the difference in bromothymol blue. Leave the wet mount of elodea under the light for 1520 minutes. Prepare a wet mount of an elodea leaf with aquarium water. An older name for this genus is anacharis, which serves. Photosynthesis in elodea lab essay example graduateway. Elodea canadensis is a submersed, aquatic, perennial plant. Classified in the frogsbit family hydrocharitaceae, elodea is native to the americas and is also widely used as aquarium vegetation and laboratory demonstrations of cellular activities. Then with the other testtube we did the same thing except we blew into it so that the btb would turn yellow. Cyclosis cytoplasmic streaming in plant cells elodea create. It starts as a blue solution in water, and turns yellow in the presence of carbon dioxide. This lab involves the qualitative measurement of the changes in carbon dioxide concentration associated with respiration and photosynthesis in the freshwater plant elodea. Sprig of elodea distilled water w baking soda test tube or clear glass timer or watch.
Elodea lab this lab is designed to show how photosynthesis takes place in the aquatic plant elodea. Elodea high school inquiryscientific method plants. We were attempting to find out how cells function, move, and act. The plant thrives in my garden pond, so the onset of the long. Prepare a wet mount of one leaf from the water plant elodea using the water in which it is kept. Video clips are shown, with tips on viewing cyclosis with a student microscopeand links to a selection of web resources describing the phenomenom. This plant may be known by one or more common names in different places, and some are listed above. Record movie and measure 5x at times 0, 2 min, 4 min control rate 3. Elodea canadensis ufifas center for aquatic and invasive. Elodea is a water plant native to canada, often used in aquariums. Antineutrophil cytoplasmic antibodies anca can occur in patients with autoimmune vasculitis including wegener granulomatosis wg, microscopic polyangiitis mpa, or organlimited variants thereof such as pauciimmune necrotizing glomerulonephritis. Record the initial color of the bromothymol blue solution.
Plus, the cells are very large so can be observed easily using a television or lcd transmission light microscope under 800. To do this, use a soaked qtip to place a drop of aquarium water towards one end of the slide. Continue measuring 5x at times 6, 8, 10, 15, 20 min rates with drug 5. In the case of plant cells with rigid cell walls, the internal contents move rat. Elodea canadensis may be confused with hydrilla verticillata or egeria densa. The motion transports nutrients, proteins, and organelles within cells. Molecular mechanisms, almost identically the similar to those that cause muscles to contract, drive chloroplast streaming. View a prepared slide of elodea anacharis, which is an aquarium plant. Cover tube 1 with tin foil there is no elodea in tube 1 and place it in the test tube rack. As the slide warms from the light of the microscope, you may see the chloroplasts moving, a process called cytoplasmic streaming.
Cytoplasmic streaming in elodea can be measured by observing the movement of chloroplasts throughout the cell. These invertebrates in turn are used as food by fish and other wildlife species e. Place the soft material from your cheek in the drop of iodine. Start with the low power objective and work your way until you have focused the leaf using the high power objective. Canadian pondweed elodea canadensis is often mentioned in biology and freshwater life books as one of the best subjects to demonstrate the phenomenom called cyclosis or cytoplasmic streaming.
You should see the chloroplasts moving in a circular pattern around the cell. Cork, onion, elodea, and frog blood lab assessment. Cytological stains are not necessary for observing the streaming of the chloroplasts. Nov 08, 2009 my lab group made up a lab in biology. Co2 concentration btb color indicator number 0 6 7 8. It creates cytoplasmic reorganization during cell reproduction. Elodea can be kept living in an aquarium long term if anchored with gravel it should then begin to grow. Plants profile for elodea canadensis canadian waterweed. Cytoplasmic streaming in plant cells elodea dic 1250x. Observe the leaf at 40x and carefully draw what you see in the space marked.
Elodea is a genus of 6 species of aquatic plants often called the waterweeds described as a genus in 1803. Elodea has no known direct food value to wildlife, but is used extensively by insects and invertebrates. Bromthymol blue is used as an indicator for the presence of co2 in solution. Using elodea in the lab science and plants for schools. We put 1 elodea plant in each graduated cylinder and poured in some bromothymol blue. Cyclosis cytoplasmic streaming in plant cells elodea. Plant cell lab onion and elodea the biology corner. This lab was meant to show us the chloroplasts moving around cells in the cytoplasm. But neither of them turned blue like we predicted and turned a swamp green is that supposed to happen. Nov 26, 2003 cytoplasmic streaming in elodea can be measured by observing the movement of chloroplasts throughout the cell. Elodea is present in virtually every region of north america kartesz, 1999.
Osmosis in elodea leaf cells predictions hypothesis experimental question results purpose if the solution that the elodea plant cells are immersed in has a higher saline concentration than the cells normal, isotonic environment, then the cells will crenate or shrink. Cytoplasmic streaming in elodea canadian journal of botany. Chloroplasts are the organelles in a plant cell that contain the chlorophyll plants use to convert light into sugars. Elodea, genus of five or six species of submerged aquatic plants in the frogsbit family hydrocharitaceae, useful in aquariums and in laboratory demonstrations of cellular activities. Observing photosynthesis this is a very simple lab that allows students to observe the formation of oxygen bubble during photosynthesis. Cork, onion, elodea, and frog blood lab assessment hochenberg. Photosynthesis and respiration in elodea experimental design sheet restate the two tasks of your experiment as hypotheses. Experiments to determine the early labeled photosynthetic products in elodea canadensis show that after 2 seconds of exposure to nah 14 co 3, 45% of the 14 c incorporated is located in malate and aspartate. I usually save the cheek cell lab for older students in anatomy class or second year biology.
It is rare in florida, occurring in some streams in jackson county. Submerged portions of all aquatic plants provide habitats for many micro and macro invertebrates. Cytoplasmic streaming cyclosis chloroplasts move throughout the cell by this function. Plasmolysis of elodea california state university, northridge. Learn vocabulary, terms, and more with flashcards, games, and other study tools. First discovered in the 1830s, the presence of cytoplasmic streaming helped convince biologists that cells were the fundamental units of life.
Short term it can be kept in a shallow tray covered with water. Cytoplasmic streaming in the higher plant elodea canadensis has been studied by light and electron microscopy. Instead of just reading about cytoplasmic streaming in a textbook, we got. Cut equalsized pieces of elodea for each test tube or glass about 3 inches in length. Measure cytoplasmic streaming in nitella and elodea test. Plant cell lab onion and elodea key plant cell, sixth. Introductionthis lab has been created in order to find what extent does distance from a light source 5cm, 10cm, and 15cm affect the rate of photosynthesis measured in bubbles 3 min in elodea water plants. Simple lab where students place elodea leaves in hypertonic solutions. Blades biological provide care information for both the pondweeds elodea and cabomba. The solution will cause an observable change in the cells due to osmosis. Gather one elodea leaf from the teacher and make a wet mount slide of the elodea.
Cell lab for introductory biology the biology corner. So a few days ago i had to do a science lab in class and it was called light photosynthesis lab. Calcium ions inhibited streaming in detached leaves, whereas magnesium ions. Click below on a thumbnail map or name for species profiles. Nitella have large cells with cytoplasmic streaming. Page includes photos of what students will observe. The plants database includes the following 5 species of elodea.
Jan 23, 2017 last week, we looked at cytoplasmic streaming of elodea leaf cells in class. Please check your test tubes and use the data to complete your data table and graph on the back of your lab sheet. Cytoplasmic streaming is the flowing of cytoplasm in eukaryotic cells. Photosynthesiselodea lab photosynthesis is the process by which plants take carbon dioxide from the atmosphere, add water, and use the energy of sunlight to produce sugar. Phosphoglyceric acid and sugars account for 27% of the label during similar exposures. The middle and upper leaves, typically three per whorl, are elliptic, approximately 25 mm wide. Mar 03, 2012 cytoplasmic streaming in plant cells elodea dic 1250x. Bromothymol blue is an indicator solution to those used to test for food nutrients. The reason for this experiment is simple in my opinion. Using forceps remove a small leaf from the tip of an elodea plant and lay it flat in the drop of water. This lab that we did was to see what would happen to see what would happen to the elodea plant if we put a different substance in our beakers.
Elodea canadensis is a dioecious, perennial, submerged aquatic macrophyte with elongated flexuous stems and long internodes which are clothed with whorls of sessile, minutelyserrate leaves and rooted from their nodes, typically in mud substrates. Photosynthesis elodea lab photosynthesis is the process by which plants take carbon dioxide from the atmosphere, add water, and use the energy of sunlight to produce sugar. Cyclosis or cytoplasmic streaming can be easily seen in cells of canadian pondweed. Nov 26, 2008 cytoplasmic streaming is the flowing of cytoplasm in eukaryotic cells. During this experiment, cells from a plant, a elodea leaf specifically, were closely examined with a light microscope. Make sure that each piece looks healthy and has plenty of green leaves. Cytoplasmic streaming in elodea canadian researchgate. Cyclosis cytoplasmic streaming in plant cells elodea on vimeo.
Last week, we looked at cytoplasmic streaming of elodea leaf cells in class. Elodea canadensis canadian waterweed this plant can be weedy or invasive according to the authoritative sources noted below. Sep 08, 2009 i usually save the cheek cell lab for older students in anatomy class or second year biology. Over all these days all the bubbles that were once there. Place a piece of elodea in each test tube or glass. Increase the magnification to 100x and refocus the slide using the fine knob. This lab can be done as a demonstration to begin the unit, or it can done as an inquiry lab where students try to devise an explanation for the bubbles they see in the test tube. Instead of just reading about cytoplasmic streaming in a textbook, we got to see it in real life.
Observe the cells under normal conditions, and make a sketch of what you see. Elodea is an freshwater, aquatic plant native to the americas commonly used in aquariums. It is also often used in biology labs on cell structure because it forms nice, large cells that are easily observed under the microscope. Tube 1 will be the control no elodea tube 2 will be the elodea in the dark. How does cytoplasmic streaming occur in elodea cells. At 400x magnification, it was truly amazing to see the chloroplasts moving in the cells. After 1520 minutes, reduce the light so that you may visualize the elodea cells. Using a clean toothpick, gently scrape the inside of your cheek. Elodea is a good model to study living plant cells in action. Elodea lab with cytoplasmic streaming and plasmolysis. Because the leaves of elodea are only a few cells thick, they will be easy to observe under the microscope to look at cells and cell parts. Make sure it is pushed down to the bottom of the test tube. While observing the leaf under the microscope, wick a solution of 6% nacl sodium chloride across the slide. Start studying cork, onion, elodea, and frog blood lab assessment hochenberg honors.
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